The effect of optimized carbon source on the synthesis and composition of exopolysaccharides produced by Lactobacillus paracasei

This study aimed to predict the optimal carbon source for higher production of exopolysaccharides (EPS) by Lactobacillus paracasei TD 062, and evaluate effect this on monosaccharide composition EPS. We evaluated EPS capacity 20 strains L. under same conditions. further investigated which showed highest EPS-producing activity (0.609 g/L), examining associated biosynthesis pathways Genomics revealed that fructose, mannose, trehalose, glucose, galactose, lactose were sources 062 could use produce identified an synthesis gene cluster participate in transport, export, sugar chain synthesis, generate 6 nucleotides. Experimental results content produced using fermentation with optimized (fructose, lactose) increased 115%. Furthermore, changed The enzyme measurements significant increases 2 key enzymes involved glycoside pathway. Our optimizing provided not only EPS, but also affected monosaccharides increasing underlying pathways, suggesting important role 062.